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Parental plants and slim1-1 and slim1-2 mutants were grown for 11 d with 1500, 300, 100, or 30 µM of sulfate (S1500, S300, S 100, and S30) or with no sulfate (S0).
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Scale bar: 100 µm. B: percentage of HA- SLIM1-transfected cells with a branched phenotype (minimum 4 cytoplasmic protrusions/cell) was compared on different surfaces: fibronectin ...
Decreased SLIM1 Expression and Increased Gelsolin Expression in Failing Human Hearts ... Either tissue homogenates (50, 25, 100, and 100 µg/lane for A, C, D, and E, respectively ...
The cells were washed in PBS and fixed and permeabilized in PBS, with 3.6% formaldehyde and 2% Triton X- 100. Preimmune or affinity-purified anti- SLIM1 sera were added to the cells ...
Large scale genetic screens have identified the gene SLIM1 as one of the most significantly ... Proc Natl Acad Sci U S A 100, 8660-5. Coghill ID, Brown S, Cottle DL, McGrath MJ, Robinson ...
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... we have cloned and characterized two genes, class 1 aldehyde dehydrogenase (Aldh1) and Slim1 ... strand synthesis was achieved by adding 40 µl of 5× second-strand synthesis buffer [ 100 ...
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A-kinase Anchoring Protein 100 (AKAP 100) is Localized in Multiple Subcellular ... Decreased SLIM1 Expression and Increased Gelsolin Expression in Failing Human Hearts ...
Other human members, FHL1/ Slim1 (25, 26) and FHL3/Slim2 , have 48 and 52% sequence identity ... This membrane-containing fraction was fractionated in the presence of 1% Triton X- 100 to ...
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... on the patterns after two d (D) on both soft (1 kPa) and stiff (8 kPa) PA gels. Bars, 100 ... Another LIM domain protein, skeletal muscle LIM protein 1 (or SLIM1), is known to induce ...
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